PCR primer and kit for screening brassica oleracea recessive cell nucleus male sterility and application thereof

The present invention develops a molecular marker and a special primer for assisting in identifying screening of brassica oleracea recessive cell nucleus male sterility (a control gene is BoMS188) andan application thereof. Nucleotide sequences of the PCR marker for identifying the brassica oleracea recessive cell nucleus male sterility are 5'-CGCATCTGATGGCAGAGATA-3' and 5'-AAGCATCTCGTCCTTGAAACA-3', and a name of the primer for detecting the PCR marker is Cufms-F/Cufms-R. The provided primer and reagents are used to assist in identifying the brassica oleracea recessive cell nucleus male sterility, and a coincidence rate with field phenotypes reaches 100%. The primer used for brassica oleracea breeding is simple in operation and easy to implement, strong in specificity and good in stability, can achieve early-period breeding, etc., and has great application prospects.