Molecular marker relevant to cauliflower waxiness and application

user-5fe1a78c4c775e6ec07359f9(2020)

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摘要
The invention discloses a molecular marker relevant to cauliflower waxiness and application. A nucleotide sequence with the molecular marker is GACTC, the GACTC is existed in a number 8 chromosome ofa normal waxiness cauliflower, a PCR primer is developed for assistant screening the cauliflower with or without the waxiness, has the advantages of co-dominance, high specificity, high stability andthe like, does not produce non-specific amplification at a temperature of 58 DEG C, and can accurately detect whether cauliflower materials to be detected are waxiness loss. A CaGL1-F/CaGL1-R is adopted to carry out the assistant screening of the waxiness loss cauliflowers, target strains can be quickly screened at a seedling stage, and breeding efficiency can be significantly improved.
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关键词
Molecular marker,Nucleic acid sequence,Primer (molecular biology),Chromosome,Genetics,Biology
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