Artificial hybridization method for millet
user-5fe1a78c4c775e6ec07359f9(2012)
摘要
The invention discloses an artificial hybridization method for millet. The method comprises the following steps: 1) for ears of maternal plants of millet with the total number of flowers accounting for 10 to 15% of the total number of spikelets, removing a part of seed strings of the ears until each of the ears has 15 to 25 seed strings and removing all the blossomed flowerets and unfully-grown flowers in the retained seed strings; 2) wetting the ears of the maternal plants processed in step 1), wrapping the ears of the maternal plants to allow the ears to be in an environment with a temperature of 23 to 30 DEG C and relative air humidity of more than 95% and carrying out emasculation and pollination after 18 to 21 h; and 3) culturing pollinated plants under the condition that the ears ofthe maternal plants are isolated from external pollen and collecting seeds. According to the invention, a method of moisture retention and emasculation through wrapping is employed; therefore, cracking of anther of the maternal plants is effectively prevented, working time for emasculation can be prolonged, and mechanical damage to stigma during manual emasculation is avoided.
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关键词
Emasculation,Pollination,Pollen,Stamen,Horticulture,Biology,Moisture retention,Relative air humidity
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