Microrna-1 (Mir-1) Activates Nf-Kappa B And Promotes The Growth Of Bone Marrow Mesenchymal Stem Cells In Glioma

miR-1 can affect the proliferation of various cancer cells but its effect on the proliferation of bone marrow mesenchymal stem cells (BMSCs) has not been reported. This study cultured BMSCs with glioma cells to investigate the effects of miR-1 on BMSCs. BMSCs were co-cultured with glioma cells using Transwell chamber, and mesenchymal stem cells (MSCs) were transfected with miR-1 mimics or miR-NC (miR-Ctrl) followed by analysis of cell proliferation by CCK8, miR-1 expression by real-time PCR, and NF-kappa B p65 protein level by Western blot. miR-1 expression and MSCs number was significantly increased after miR-1 mimics transfection. Cell number in experimental group and blank group was significantly elevated compared to control group (P < 0 05). NF-kappa B p65 protein expression in BMSCs in control group and experimental group was also significantly increased compared to miR-Ctrl and blank group with higher level in experimental group than control group (P < 0.05). miR-1 can promote the proliferation of BMSCs by activating NF-kappa B. The glioma cell co-culture microenvironment can further promote its proliferation.