Gas Chromatography-Mass Spectrometry Analysis of 11-Nor-9-carboxy-Δ9-Tetrahydrocannabinol in Urine
Journal of Food and Drug Analysis(2020)
摘要
Effective specimen pretreatment and GC-MS protocols were developed for the analysis of 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THC-COOH) in urine. d3- and d9-11-nor-9-carboxy-Δ9-tetrahydrocannabinol (d3- and d9-THC-COOH) were evaluated as internal standards and conclusions were reached that THC-COOH can be effectively analyzed, qualitatively and quantitatively, as trimethylsilyl derivative using selective ion monitoring of the following ions: m/z 371, 473 and 488 (for THC-COOH) and m/z 380, 479 and 497 (for d9-THC-COOH). The extraction procedure achieved 90% recovery of the analyte in fortified drug-free urine containing 10-200 ng/ml THC-COOH. Using 2-ml sample size, the overall protocol resulted in the following analytical parameters in the 10-200 ng/ml concentration range studied: interday and intraday precision ranges, 0.35-5.31% and 0.44-5.19%, respectively; linearity, r2 > 0.999.
更多查看译文
关键词
Drug Analysis,Toxicity Assessment
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要