Evaluation The Antioxidant Activity Of Syrian Ficus And Olive Leaf Extracts And Their Inhibitory Effects On Alpha-Glucosidase In Vitro

In this work we examined the effectiveness of ethanolic and aqueous extracts of Syrian ficus leaf and their mixes with Syrian olive leaf extracts (1:1, 5:1, 7:1) (olive: ficus) in inhibition of both of 2,2-diphenyl-1 picrylhydrazyl (DPPR), 2.2,-azino-bis-[3-ethyl benzothiazoline-6-Sulfonic Acid] (ABTS(+.)). The study also investigated the inhibition of both of ficus and olive leaf extracts and their mixe (1:1) on alpha-glucosidase in order to investigate the ability of these extracts to reduce blood sugar in vitro. The results showed that there were significant differences in the ability of the mixes to inhibit DPPR, the lower IC50 value was 0.24 +/- 0.01 mg/ml for the mix (1:1), as oppose to the results with the ABTS(+.), there is no significant differences in the ability of the mixes (1:1, 5:1) and ethanolic olive leaf extract to inhibit ABTS(+.), (IC50 =0.47 +/- 0.01 mg/ml). The Inhibition percentage of alpha-glucosidase reached 98.5% at 7.69 mg/ml and 81.34% at 3.85 mg/ml for aqueous ficus and ethanolic olive leaf extracts, respectively. The ethanolic olive leaf extract gave the lower value (IC50 = 0.34 +/- 0.12 mg/ml). The alpha-glucosidase inhibition mode was also tested, the max velocity V-max and Micheles-Menten constant K-m were calculated from the Lineweaver-Burk equation. The result showed that inhibition modes were non-competitive and competitive types for ficus and olive leaf extracts, respectively.