Investigation of the limit of discovery using tile-based Fisher ratio analysis with comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry

•The discovery limit (DL) of tile-based F-ratio analysis depends on the analyte LOQ•JP8 jet fuel spiked with sulfur-containing compounds was studied•F-ratio hitlist rankings using average F-ratio versus top F-ratio were compared•Rankings of low concentration analytes approaching DL improved using top F-ratio•Hitlist rankings worsen with an overly large or small F-ratio tile size