3D printed alginate bead generator for high-throughput cell culture

Alginate hydrogel beads are a common platform for generating 3D cell cultures in biomedical research. Simple methods for bead generation using a manual pipettor or syringe are low-throughput and produce beads showing high variability in size and shape. To address these challenges, we designed a 3D printed bead generator that uses an airflow to cleave beads from a stream of hydrogel solution. The performance of the proposed alginate bead generator was evaluated by changing the volume flow rates of alginate ( Q Alg ) and air ( Q A ), the diameter of device nozzle ( d ) and the concentration of alginate gel solution ( C ). We identified that the diameter of beads ( D = 0.9 -2.8 mm) can be precisely controlled by changing Q A and d . Also the bead generation frequency ( f ) can be tuned by changing Q Alg . Finally, we demonstrated that viability and biological function (pericellular matrix deposition) of chondrocytes were not adversely affected by high f using this bead generator. Because 3D printing is becoming a more accessible technique, our unique design will allow greater access to average biomedical research laboratories, STEM education and industries in cost- and time-effective manner.