Variations in root morphological indices of rice (Oryza sativa L.) induced by seedling establishment methods and their relation to arsenic accumulation in plant tissues

Understanding how the seedling establishment method affects arsenic (As) accumulation in rice is important for safe agricultural production. In a field experiment with three seedling establishment methods and two rice cultivars, the effects of direct seeding (DS), manual transplanting (MT), and seedling throwing (ST) on root morphological indices and the distribution, translocation, and accumulation of As in rice tissues across growth stages were compared. DS method resulted in the greatest accumulation of As in the two rice cultivars and led to more As distributed in aboveground tissues during the entire growth period. Especially in DS brown rice, the concentration of total As increased by 24.0%–40.8%, and that of inorganic As increased by 24.4%–40.0%, compared with the concentrations in MT and ST rice. A multiple regression model was developed with root morphological indices and the total As concentration in brown rice, and the R2 value of the model was 0.819, which was significant at the 1% level. Compared with the other establishment methods, the thinner diameters, smaller volumes, larger specific surface areas, and greater numbers of root tips in DS rice roots across growth stages promoted As uptake. The concentrations of As in root tips were approximately five times greater than those in the root base, and root tips were the key factor determining the difference in As accumulation in rice roots under the different seedling establishment methods. The results of this study demonstrate that the choice of an appropriate rice seedling establishment method is important to avoid the environmental consequences of As contamination and safely produce rice grain. Therefore, despite the current trend of increased use of DS, transplantation is recommended as a safer pattern of rice seedling establishment in As-contaminated areas.