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Method of producing nanobiocatalysts

user-5f8cf7e04c775ec6fa691c92(2014)

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Abstract
A method of producing nanobiocatalysts comprises: suspending a support material in a liquid medium; functionalizing a surface of the support material by mixing the suspended support material with a functionalizer for a first predefined time at a first predefined temperature wherein a first broth results; applying a protein directly in the first broth after the first predefined time; mixing the protein with the first broth and incubating the enzyme for a second predefined time at a second predefined temperature wherein a second broth results; immobilizing the incubated protein onto the functionalized surface of the support material wherein a third broth results; filtering the third broth; and gathering a retentate of the filtering. By directly processing the first and second broths the method according to the invention allows for providing a continuous process and production of the nanobiocatalysts. Thereby, the production can be optimized regarding time, water, chemical and energy consumption. Also, the method according to the invention allows for a nanobiocatalysts production in which problems related to thixotrophy can be minimized. These effects can drastically increase the efficiency of the process thereby making production of nanobiocatalysts at a comparably high rate possible. Furthermore, the method according to the invention also allows for saving resources such that efficiency can also be increased in this regard.
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Key words
Ultrafiltration,Energy consumption,Process engineering,Chromatography,Chemistry,A protein,High rate,Liquid medium
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