[The role of Bmal1 in regulating the aging of mesenchymal stem cells via Wnt/β-catenin signaling pathway].

PURPOSE:To investigate whether Bmal1 and Wnt/β-catenin signaling pathway has synergistic or antagonistic effects on the aging of mouse bone marrow-derived mesenchymal stem cells (BMSCs). METHODS:The cells were divided into two groups. The expression of β - catenin and TCF1 in the transfected group and the blank group were detected by real-time fluorescent quantitative PCR and Western blot. The data were statistically analyzed using SPSS 19.0 software package. RESULTS:Recombinant Bmal1 lentiviral vector was successfully constructed. The expression of β-catenin was enhanced after Bmal1 was transfected(P<0.05), which was different from that of TCF1. There was no significant difference in TCF1. CONCLUSSIONS:Bmal1 has a synergistic effect on the aging changes of MSCs in mice directly or indirectly regulated by Wnt/β-catenin signaling pathway.