De Novo Histidine Biosynthesis Protects Mycobacterium Tuberculosis From Host Ifn-Gamma Mediated Histidine Starvation

Intracellular pathogens including Mycobacterium tuberculosis (Mtb) have evolved with strategies to uptake amino acids from host cells to fulfil their metabolic requirements. However, Mtb also possesses de novo biosynthesis pathways for all the amino acids. This raises a pertinent question- how does Mtb meet its histidine requirements within an in vivo infection setting? Here, we present a mechanism in which the host, by up-regulating its histidine catabolizing enzymes through interferon gamma (IFN-gamma) mediated signalling, exerts an immune response directed at starving the bacillus of intracellular free histidine. However, the wild-type Mtb evades this host immune response by biosynthesizing histidine de novo, whereas a histidine auxotroph fails to multiply. Notably, in an IFN-gamma (-/-) mouse model, the auxotroph exhibits a similar extent of virulence as that of the wild-type. The results augment the current understanding of host-Mtb interactions and highlight the essentiality of Mtb histidine biosynthesis for its pathogenesis. Dwivedy and Ashraf et al. use a hisD knockout strain of M. tuberculosis to show that the mouse immune system controls and eliminates the hisD knockout strain by inducing an IFN gamma -dependent depletion of host histidine. These results suggest a link between histidine metabolism and the pathogenesis of M. tuberculosis infection.