富含ω-3多不饱和脂肪酸的磷脂酰丝氨酸制备工艺研究

LIAO Miaofei,FU Wandong,SUN Jipeng,ZHOU Yufang,MA Mingzhu,WANG Jiaxing, YAO Wei, HU Shiqi

Journal of Fisheries Research（2022）

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Abstract

本研究以南极磷虾油、L-丝氨酸为原料,在磷脂酶D(Phospholipase D-catalyzed,PLD)的催化作用下发生转酰基反应,制得富含二十碳五烯酸(Eicosapentaenoic acid,EPA)、二十二碳六烯酸(Decosahexaenoic acid,DHA)的磷脂酰丝氨酸(Phosphatidylserine,PS).通过单因素和正交试验,确定了PS的最佳制备工艺条件:以乙酸乙酯为有机溶剂、水相pH 4.5、有机相与水相体积比1:1、反应温度40℃、加酶量55U、L-丝氨酸与南极磷虾油质量比16:1、反应时间8 h,在此条件下,PS得率为85.62％,产物中EPA含量为24.33％,DHA含量为19.95％.

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