Laboratory Demonstration Of The Vertical Transmission Of Rift Valley Fever Virus By Culex Tarsalis Mosquitoes

Rift Valley fever virus (RVFV) is a mosquito-transmitted virus with proven ability to emerge into naive geographic areas. Limited field evidence suggests that RVFV is transmitted vertically from parent mosquito to offspring, but until now this mechanism has not been confirmed in the laboratory. Furthermore, this transmission mechanism has allowed for the prediction of RVFV epizootics based on rainfall patterns collected from satellite information. However, in spite of the relevance to the initiation of epizootic events, laboratory confirmation of vertical transmission has remained an elusive research aim for thirty-five years. Herein we present preliminary evidence of the vertical transmission of RVFV by Culex tarsalis mosquitoes after oral exposure to RVFV. Progeny from three successive gonotrophic cycles were reared to adults, with infectious RVFV confirmed in each developmental stage. Virus was detected in ovarian tissues of parental mosquitoes 7 days after imbibing an infectious bloodmeal. Infection was confirmed in progeny as early as the first gonotrophic cycle, with infection rates ranging from 2.0-10.0%. Virus titers among progeny were low, which may indicate a host mechanism suppressing replication.Author summaryRift Valley fever virus (RVFV) represents a significant threat in terms of its ability to emerge into naive geographic areas. Furthermore, RVFV represents a global public health risk due to the ability of many mosquito species to transmit the virus and the ease with which the virus can be transported due to increased globalization. The vertical transmission of RVFV by mosquitoes has long been accepted by the research community due to limited field evidence. However, laboratory confirmation of vertical transmission has remained elusive for thirty-five years. We present the first laboratory evidence of vertical transmission of RVFV in the susceptible North American vector, Culex tarsalis. We present two studies that clearly show 1) the accumulation of RVFV antigen in the ovaries of infected mosquitoes and 2) the transmission of RVFV from parent to offspring immediately following an infectious blood meal.