A high-throughput and low-cost maize ear traits scorer

In this study, based on automatic control and image processing, a high-throughput and low-cost maize ear traits scorer (METS) was developed for the automatic measurement of 34 maize ear traits. In total, 813 maize ears were measured using METS, and the results showed that the square of the correlation coefficient ( R 2 ) of the manual measurements versus the automatic measurements for ear length, ear diameter, and kernel thickness were 0.96, 0.79, and 0.85, respectively. These maize ear traits could be used to classify the type, and the results showed that the classification accuracy of the support vector machine (SVM) model for the test set was better than that of the random forest (RF) model. In addition, the general applicability of the image analysis pipeline was also demonstrated on other independent maize ear phenotyping platforms. In conclusion, equipped with image processing and automatic control technologies, we have developed a high-throughput method for maize ear scoring, which could be popularized in maize functional genetics, genomics, and breeding applications.