Effects of Different Gelling Agents and Different Doses of Plant Growth Regulators for Callus Induction under In vitro Culture of Banana (Musa paradisiaca L.) Variety Udhayam

Present investigation “Effects of different gelling agents and different doses of plant growth regulators for callus induction under in vitro culture of Banana (Musa paradisiaca L.) variety Udhayam” was carried out at the Tissue Culture Laboratory Department of Horticulture, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut during the year 2018-2019. Different type of gelling agents as Tapioca starch, Corn starch, Asparagus and Agar to develop efficient callus inductions. The minimum time of callus induction 25.53 days was observed in treatment Tapioca starch @ 60 gl-1.The effect of different concentration of gelling agents and 2,4-D for the callus induction. Minimum time of callus induction for Tapioca starch was observed 25.81 days with the treatment of 2,4-D @ 4.00 mg-1 + Kinetin 1.00 mg-1.The earliest time of callus induction was noted 26.57 days for Corn starch with the treatment of 2,4-D @ 4.00 mg-1 + Kinetin 1.00 mg-1.Asparagus showed that significantly minimum time of callus induction 28.38 days was observed in treatment 2,4-D @ 4.00 mg-1 + Kinetin @ 1.00 mg-1 with MS media. Significantly minimum time of callus induction 30.73 days was observed for Agar in the treatment 2,4-D @ 4.00 mg-1 + Kinetin 1.00 mg-1 with MS media.