Laser Ablation-Single-Particle-Inductively Coupled Plasma Mass Spectrometry As A Multimodality Bioimaging Tool In Nano-Based Omics

Advanced data processing and visualization techniques were applied to laser ablation-single particle-inductively coupled plasma mass spectrometry (LA-sp-ICPMS) data obtained from imaging of roots of sunflower (Helianthus annuus L.) which were exposed to ionic silver (Ag+). Highly multiplexed images with tissue level resolution were generated, showing the uptake and transformation of Ag . to silver nanoparticles (AgNPs) in whole root cross-sections, and revealing that the size of biosynthesized AgNPs is predisposed by the reducing power of particular root compartments. Various Ag divided by and AgNP visualization strategies are shown with special emphasis on spatially resolved information associated with the number and spherical-equivalent size of individual AgNPs in user-selected root cross-sectional regions, including statistical analysis of the AgNP distribution. Normally, this multimodality information can only be obtained by combining the results of techniques from several complementary platforms.