Selection of specific nanobodies to develop an immuno-assay detecting Staphylococcus aureus in milk

Yaozhong Hu,Ying Sun,Jiaxin Gu,Feier Yang,Sihao Wu,Chuan Zhang,Xuemeng Ji,Huan Lv,Serge Muyldermans,Shuo Wang

Food Chemistry（2021）

引用 43|浏览15

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摘要

•The first report ever on nanobodies directed against intact S. aureus.•A Nb-mediated immuno-assay avoids false-positive detection of S. aureus.•Development of a sensitive sandwich ELISA against S. aureus.•The potential application to detect intact S. aureus in food matrix.

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关键词

Staphylococcus aureus,Food-borne pathogen,Nanobody,Sandwich ELISA,Immuno-assay

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