Lacticaseibacillus Absianus Sp. Nov., Isolated From The Cecum Of A Mini-Pig

A rod- shaped, facultative anaerobic, Gram- stain- positive bacteria, isolated from the cecum of a mini- pig, was designated as strain YH-lac23T. Analysis of 16S rRNA gene sequences revealed that the strain was closely related to Lacticaseibacillus daqingensis JCM 33273T (97.9 %), Lacticaseibacillus porcinae KCTC 21027T (96.2 %) and Lacticaseibacillus manihotivorans KCTC 21010T (95.7 %). Analysis of housekeeping gene sequences (pheS and recA) revealed that the strain formed a sub- cluster with L. daqingensis. The average nucleotide identity value for YH- lac23T and its most closely related strain (L. daqingensis) is 80.7 %. The main fatty acids are C18:1?9c and C16:0. The cell wall contains the peptidoglycan of meso- diaminopimelic acid. The G+C content of the genomic DNA is 59.8 mol%. In view of the chemotaxonomic, phenotypic and phylogenetic properties, YH- lac23T (=KCTC 25006=JCM 33998) represents a novel taxon. The name Lacticaseibacillus absianus sp. nov. is proposed.