Resolution Improvement Of Dark-Field Microscopy Via Microparticle Near-Field Illumination

OPTICS LETTERS(2021)

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摘要
In this Letter, a novel, to the best of our knowledge, approach to improve the imaging resolution of dark-field microscopy is proposed and demonstrated. Inspired by an existing super-resolution imaging method based on near-filed illumination using a prism or microfiber, a microparticle-generated full-direction evanescent field for sample illumination was demonstrated to achieve a multi-orientation near-field illumination in one snapshot and to obtain a super-resolution image by spatial frequency shifting. The ultimate resolution and the additional magnification factor of this method were analyzed theoretically. Imaging experiments were carried on a standard microscope calibration target MetroChip and a Blu-ray disc characterized by subwavelength microstructures. Highimaging resolution was demonstrated experimentally, and two novel illumination modes were proposed to overcome imaging direction selectivity. Our work opened up a new perspective of super-resolution imaging with near-field illumination. (C) 2021 Optical Society of America
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关键词
microparticle,illumination,resolution,dark-field,near-field
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