1D continuous gel electrophoresis composition for the separation of deamidated proteins

Axel Boudier-Lemosquet,Adrien Mahler,Claude Bobo,Violaine Moreau,Muriel Priault

Methods（2022）

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摘要

•Rapid affordable continuous gel for electrophoretic screening of demidable proteins.•Eectrophoretic separation of native and deamidated forms of proteins.•Multifactorial screening to improve the formulation of protein storage buffer.

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关键词

Denaturing electrophoresis,Separation of deamidated proteins,Buffer formulation,Protein stability,Bcl-xL,Calmodulin,RhoA,Rac1,Cdc42

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