Rapid HIT 200在法医DNA检验中的应用
Forensic Science and Technology(2016)
Abstract
目的:检验Rapid HIT 200系统快速检测DNA的法医学可应用性。方法将血斑、唾液斑、脱落细胞及各种组织以该系统检测,从灵敏度、适应性、重复性和防污染等方面评估其效能。结果该系统检测全血样本(血液)灵敏度为1μL,模板量30 pg左右;血斑、组织与唾液斑的检出率均在80%以上,但对脱落细胞仅为16%;对测试的100个样本中,未发生样本自身或彼此间的转移污染;对血斑、唾液斑、脱落细胞的重复性检验,其基因座一致性检出率达90%以上。结论该系统具备防污染能力,对常量检材的检测能力优于微量。
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Key words
Rapid HIT 200 apparatus,STR loci,sensitivity,adaptability,preventability against contamination,reproducibility
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