马鼻狂蝇幼虫感染的蒙古马咽部细胞和体液免疫研究

Journal of Agricultural Science and Technology(2018)

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Abstract
为了探索马鼻狂蝇幼虫感染的细胞和体液免疫情况,选取在呼和浩特屠宰场收集到的8例马鼻狂蝇幼虫感染的蒙古马为感染组,以5匹健康蒙古马为对照组.马咽部切片HE(haematoxylin and eosin)染色发现感染组马的咽部巨噬细胞、嗜酸性粒细胞、肥大细胞增多,咽部黏膜增生、坏死,出现不同程度增生性炎(2龄和3龄幼虫寄生处).通过对感染组马鼻腔分泌黏液、血液间接酶联免疫吸附测定法(enzyme linked immunosorbent assay,ELISA)和咽部组织免疫组化方法检测,发现在感染组马鼻腔分泌的黏液和血清中有大量特异性IgG抗体;马咽部出现大量分泌IgG阳性细胞.初步探索了马鼻狂蝇幼虫感染马的病理变化和免疫机制.
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Key words
Rhinoestrus spp.,cellular immunity,humoral immunity,immunohistochemistry,pharyngeal
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