羽毛降解菌株的筛选及其发酵条件的优化
wf(2016)
Abstract
从长期堆积腐烂鸡毛的土壤中分离、筛选、纯化出1株能够高效降解羽毛角蛋白的菌株。该菌为杆状、内生孢子、革兰氏染色阳性;以羽毛为唯一的碳源和氮源,研究了发酵条件对该菌株降解羽毛生产可溶性蛋白的影响,通过对单因素实验及正交优化试验,确定了该菌株最佳发酵条件为3%的接种量时,添加6 g/150 m L的羽毛量、初始p H 11.0、30℃下摇床发酵7 d后,获得的发酵液中可溶性蛋白最大浓度为34.375 g/L。
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Key words
feather,degradation,isolated strain,fermentation conditions,orthogonal optimization
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