Chrome Extension
WeChat Mini Program
Use on ChatGLM
VIPCreated with Sketch.

旱柳在Pb、Cd胁迫下实时荧光定量PCR内参基因的筛选

ZENG Jing,WANG Ping,SUN Jikang,YANG Lanpeng,ZHOU Tao,RONG Jian

Journal of Central South University of Forestry & Technology（2017）

Cited 0|Views4
No score
Abstract
为筛选适用于Pb与Cd胁迫下旱柳实时荧光定量PCR的内参基因,应用荧光定量PCR技术分析UBQ1、TUB6、Actin、EF1a、18SRNA、GAPDH、TUA8和UBQ2这8个内参基因在旱柳根部的表达情况.通过Normfmder、geNorm和Best Keeper3个软件进行综合分析,发现在Pb与Cd胁迫下表达较为稳定的内参基因均为EF1a、GAPDH和Actin,差别是在Pb胁迫下的旱柳根组织中最为稳定的内参基因是EF1a,而在Cd胁迫下的旱柳根组织应最稳定的内参基因为GAPDH.该研究结果可用于旱柳根组织在Pb、Cd胁迫下基因表达的进一步研究.
More
Translated text
Key words
reference gene,quantitative real-time PCR,cadmium,lead
AI Read Science
Must-Reading Tree
Example
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined