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流行性出血热病毒的细胞培养

王美亮, 彭静,王超美, 高珊,周学良

JOURNAL OF LANZHOU UNIVERSITY(UATURAL SCIENCES)（2001）

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Abstract
用灭活的流行性出血热病毒(EHFV)L99株感染传代培养的Vero-E6细胞,第4 d 50%的Vero-E6细胞胞浆内可查到对EHFV鼠血清的特异性荧光,第7～10 d阳性细胞数达到 100%.用感染第3 d的细胞培养上清大批量盲种,第7～10 d荧光强度均达到++++.电镜下病毒呈圆形或卵圆形,外膜为电子致密层,界面清楚,包绕着比较疏松的内浆,内浆中有若干个颗粒丝状结构.
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