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Pin1的细胞定位调控Rb蛋白的磷酸化状态

Rui-Hong LIU,Gang XIE,Zhi-Xiong XIAO,Ying TONG

Journal of Sichuan University(Natural Science Edition)（2016）

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Abstract
为了研究Pin1和Rb蛋白的细胞定位、Pin1调控Rb磷酸化的相关机制,本研究构建了慢病毒载体pLVX-Flag-HA-Pin1和Plko.1-shRNA,包装病毒感染人非小细胞肺癌(H1299),免疫印迹检测Pin1蛋白表达水平,采用免疫荧光染色、免疫组化技术研究蛋白的定位.结果表明,沉默Pin1可降低Rb的磷酸化并抑制细胞的增殖;培养的肿瘤细胞和肿瘤组织中,Pin1可定位到细胞质中,而胞质定位的Pin1也可增加Rb的磷酸化.
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Key words
Pin1,Retinoblastoma protein,cellular localization,phosphorylation
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