靶向沉默人HER2/neu基因的RNA干扰

Li-yan LEI,Xiao-li SUN, Li-min ZHU,Zhi-ming XIU,Li-ping WANG

Journal of Jilin University(Science Edition)（2013）

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Abstract

针对人HER2/neu基因的mRNA序列,设计并构建shRNA干扰载体,经聚合酶链式反应(PCR)及测序鉴定后转染SK-BR-3细胞,并检测HER2/neu mRNA和蛋白抑制情况及SK-BR-3细胞迁移能力的变化.实验结果表明:构建了3个HER2/neu-shRNA真核表达质粒;3个重组质粒均可不同程度下调HER2/neu mRNA和蛋白表达水平,并抑制SK-BR-3细胞的迁移;其中1号重组质粒效应最强,对mRNA和蛋白的抑制率分别为84.65％00和74.98％.

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Key words

HER2/neu,breast cancer,shRNA interfering vector

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