海生红藻多管藻中藻胆蛋白的分离纯化

Journal of Yantai University (Natural Science and Engineering Edition)(2015)

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Abstract
选用Middle Fine Sephadex G-150( MFG-150)、Fine Sephadex G-150( FG-150)以及Sephacryl S-300(S-300)3种凝胶过滤对富含R-藻红蛋白(R-PE)的多管藻藻胆蛋白提取液进行R-PE、R-藻蓝蛋白( R-PC )和别藻蓝蛋白( AP )的分离,并对所得R-PE、R-PC/AP组分用DEAE Sepharose-Fast Flow离子交换层析做进一步纯化,优化建立了从多管藻藻胆蛋白提取液中同时有效分离纯化R-PE、R-PC和AP 3类藻胆蛋白组分的技术方法。
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Key words
chromatography,polyacrylamide gel electrophoresis,Polysiphonia urceolata Grev,phycoerythrin,phy-cocyanin,allophycocyanin
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