番茄ACC合成酶基因的反义RNA-核酶嵌合基因植物表达载体的构建及对番茄的转化
ACTA SCIENTIARUM NATURALIUM UNIVERSITATIS NEIMONGOL(2001)
Abstract
将克隆的番茄ACC合成酶基因的反义RNA-核酶嵌合的DNA序列用HindⅢ和KpnI切割后重组于植物表达载体pGA643中,用三亲融合法导入农杆菌LBA4404中,采用叶盘法转化番茄子叶,诱导出再生小植株,获得了卡那霉素的抗性植株.提取植物总DNA,用pGA643作探针,通过斑点杂交,已筛选出整合有外源基因的工程植株.为进一步研究该嵌合基因对ACC合成酶基因表达的影响及获得商品化的转基因番茄奠定了基础.
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