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利用激光共聚焦显微镜观察骨骼肌肌纤维中的钙火花现象

Yong-ji YANG,Ying TANG,Hui-e WANG,Qiang XIAO, Jie HUANG

JOURNAL OF CHINESE ELECTRON MICROSCOPY SOCIETY（2005）

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Abstract
钙火花(Ca2+sparks)现象是近年来在骨骼肌、心肌兴奋收缩偶联研究中发现的重要生理现象,但目前确切的机制还不清楚.我们利用激光扫描共聚焦显微镜对经Flou-3 AM染色的人工剥离的蟾蜍骨骼肌肌纤维进行连续扫描分析,在骨骼肌肌纤维中捕获到钙火花现象,并对钙火花现象的发生进行了初步探讨.
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Key words
skeletal muscular fibers,confocal microscope,sparks,ca~2+
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