农药克菌丹多克隆抗体的制备与鉴定
Journal of Instrumental Analysis（2012）
Abstract
制备针对农药克菌丹的多克隆抗体,以此为基础建立该农药的快速免疫筛选检测方法.从THPI(Tetrahydrophthalimide)和Phthalimide出发合成农药克菌丹半抗原.使之与牛血清白蛋白共价偶联,合成免疫原.偶联成功后的免疫原用于免疫新西兰白兔.从白兔耳缘静脉采血,辛酸-硫酸铵法纯化多克隆抗体.间接竞争ELISA法测定其对半抗原的灵敏度、特异性和亲和性.结果显示,抗体效价为2.38×105,IC50为2μg·mL-1,灵敏度分别为12.5 ng· mL-1,25.0 ng·mL-1,显示此抗克菌丹的多克隆抗体亲合力高、特异性强,为建立克菌丹简便、快速、特异的免疫检测方法奠定了基础.
MoreKey words
captan,polyclonal antibodies,immunochemical assays
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