Development Of Correlative Super-Resolution Fluorescence And Three-Dimensional Topography Imaging Microscopy

A correlative super-resolution fluorescence and three-dimensional (3D) topography imaging microscopy was built, and the performance of the correlative system was evaluated with F-actin as an example. The experimental results suggested that the correlative instrument had the abilities to provide 3D overall topography and distribution of specific components at nanometer resolution, as well as to correlate the specific components' distribution with the overall topography. Thus, the correlative instrument is a powerful tool to investigate events regarding cytoskeleton and cellular substructure such as the composition and distribution characteristics of membrane protein clusters, and can also be widely applied to other fields such as life science and material science.