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2,6-dansyl azide快速测定血清中H2S含量

FU Lin,ZOU Xiao-yan,MA Jian-bin,DU Yu-rong,GUO Song-chang

Chinese Journal of Analysis Laboratory（2018）

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Abstract
对测定血清中H2 S浓度的方法进行了改进.基于2,6-dansyl azide本身不发荧光,但与H2 S作用后显示强烈荧光这一特性,一步法合成2,6-dansyl azide后采用PBS/DMSO体系(pH 7.5)替代PBS/Tween-20体系(pH 7.5)测定内源性H2 S.数据分析显示改进的方法具有更低的荧光背景值.应用本方法成功对6只成年健康SD大鼠的血浆H2 S水平进行了测定.
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