超高效液相色谱-串联质谱法检测血液斑痕中磺酰脲类降糖药
Chinese Journal of Analysis Laboratory(2017)
Abstract
建立了血液斑痕中格列本脲,格列美脲,格列吡嗪的液相色谱-质谱检验法.选用ACQUITY UPLC HSS C18(2.1×50 mm,1.8 μm)色谱柱,使用0.1%甲酸水-乙腈体系作为流动相进行梯度洗脱.采用ESI+电喷雾离子源,多反应监测扫描模式(MRM)检测3种磺酰脲类降糖药.在最优条件下,磺酰脲类降糖药在1.25 ~ 250 ng/patch (swab)范围内有良好线性关系,保留时间依次为2.90,3.00,2.34 min.检出限为0.1 ng/mL.渗透性客体上药物的回收率随着浓度的增大而增大,非渗透性客体上药物的回收率随着浓度的增大而减小.日内与日间精密度均小于15%,基质效应在0.85 ~ 1.15之间.可用于血液斑痕中格列本脲,格列美脲,格列吡嗪的快速筛查.
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Key words
Blood spots,Sulfonylureas hypoglycemic agents,UPLC-MS
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