液相色谱-串联质谱法测定浓缩樱桃李汁中罗丹明B和苏丹红染料
Journal of Analytical Science（2014）
Abstract
建立了液相色谱-串联质谱(HPLC-MS/MS)法同时检测浓缩樱桃李汁中罗丹明B和苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ5种染料的分析方法.样品经水稀释、乙腈提取,凝胶渗透色谱(GPC)净化,采用Zorbax Eclipse Plus C18色谱柱进行分离.在电喷雾正离子模式下(ESI+),用多重反应监测(MRM)方式进行离子监测,基质匹配外标法定量.5种染料在各自线性范围内,相关系数(r2)均大于0.998,检出限(信噪比=3)为0.2～3.0μg/kg,定量限(信噪比=10)为0.5～10.0 μg/kg,回收率在74.4％～95.3％之间,相对标准偏差为3.6％～7.9％.
MoreKey words
Gel permeation chromatography,Rhodamine B,Sudan,High performance liquid chromatography-tandem mass spectromery
AI Read Science
Must-Reading Tree
Example
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined