胡蜂蜂毒肽的固相合成
Chinese Journal of Synthetic Chemistry(2020)
Abstract
采用Fmoc固相合成策略,合成了胡蜂蜂毒肽(COOH-Ile-Asn-Leu-Lys-Ala-Leu-Ala-Ala-Leu-Ala-Lys-Lys-Ile-Leu-NH2).以Wang树脂为载体,HBTU-HOBt为缩合剂,按照其氨基酸序列依次缩合,最终用切割试剂将其从树脂上切割下来,得到粗肽,经RP-HPLC纯化得到目标肽,纯度97.6%.经HR-MS (EI)分析,确定产物为胡蜂蜂毒肽.
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