Chrome Extension
WeChat Mini Program
Use on ChatGLM
VIPCreated with Sketch.

大肠杆菌Small RNA EsrE的转录调控

Jie SONG,Bing-Bing HOU,Jiang YE,Hai-Zhen WU,Hui-Zhan ZHANG

Microbiology（2020）

Cited 0|Views4
No score
Abstract
[背景]大肠杆菌中Small RNA EsrE调控琥珀酸脱氢酶的表达并影响细胞生长,对其调控机制的探究有利于加深EsrE对细胞生长影响的认识.[目的]探究大肠杆菌Small RNA EsrE的转录调控机制.[方法]通过双质粒报告系统筛选转录调控因子,并通过凝胶迁移实验(electrophoretic mobility shift assay,EMSA)和qRT-PCR研究方法验证转录调控因子.[结果]双质粒报告系统证明RNA聚合酶亚基σ32 (RpoH)上调PesrE,β-羟酰-ACP脱水酶(FabZ)下调PesrE.EMSA结果和体内实验显示RpoH结合PesrE片段,FabZ不结合PesrE片段.[结论]RpoH直接结合启动子序列参与调控,FabZ以其他方式间接参与Small RNA EsrE的转录调控.
More
AI Read Science
Must-Reading Tree
Example
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined