通过获得链霉素抗性基因突变株筛选小诺霉素高产菌株
MICROBIOLOGY（2004）
Abstract
通过链霉素对小诺霉素产生菌(Micromonospora.purpura)49-12测定,采用诱变剂EMS 3种不同诱变剂量对菌株的孢子进行诱变处理,诱变处理的孢子涂布在含链霉素致死浓度的改良高氏平板上,获得大量的链霉素抗性基因突变株,然后从链霉素抗性基因突变株进一步筛选小诺霉素高产菌株,获得小诺霉素菌株49-12-3菌株.在摇瓶条件下,其产小诺霉素生物活性单位比出发菌株49-12#的摇瓶发酵单位提高了40%以上.小诺霉素的组分比由出发菌株的C2b:C1a的5:5提高到8:2.C2b有效组分提高了30%;链霉素抗性基因突变与小诺霉素发酵单位突变之间,小诺霉素正突变率达到40%,负突变率达26%,正突变大于负突变.
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