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棘托竹荪菌盖蛋白提取及分析

Meng CAO,Chen-qiang LIN,Rong-bin LIN,Ji-chen CHEN,Hong-duan QIU,Xin-jian LIN

Natural Product Research and Development（2014）

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Abstract
首次以棘托竹荪菌盖为原料,通过单因素试验和正交试验,研究菌盖蛋白最佳提取工艺;用SDS-PAGE测定所提取蛋白的分子量分布.结果表明:提取时间和pH是影响竹荪菌盖蛋白提取的主要因数,最佳提取条件为pH8.0,提取时间2.5h,提取温度55℃,提取率达64.07％,提取的竹荪菌盖主要蛋白分子量为16 kDa和38 kDa.棘托竹荪菌盖作为蛋白提取原料是可行的,以其为原料开发蛋白相关产品具有巨大的潜力和广阔的市场前景.
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Key words
Dictyophora echinovolvata pileus,protein,extraction
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