梳棉状嗜热真菌发酵产耐热木聚糖酶培养条件的优化
Genomics and Applied Biology(2017)
Abstract
通过多个单因素实验对该菌株发酵产酶的培养条件进行优化,包括碳源、氮源、无机盐、培养基起始pH、培养时间以及培养温度等因素,得出了一个适合该嗜热真菌产酶发酵的条件:麸皮浓度为4% (w/v)、牛肉膏浓度为0.5% (w/v)、NaCl浓度为0.25% (w/v)、K2HPO4浓度为0.2% (w/v)、CaCl2浓度为0.02% (w/v).培养基初始pH为7.0,装液量为60 mL,发酵温度为55℃,发酵时间为60 h,摇瓶转速为125 r/min.优化之后酶活可达到68.5 U/mL,比未优化之前提高了71.2%.嗜热真菌可以利用麸皮、玉米芯粉等农业废弃物作为能源产木聚糖酶,既可以很好地处理此类农业副产品,又可以获得对人类有利的产物.
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Key words
Thermophilic fungi,Xylanase,Optimization of culture conditions
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