实时荧光定量PCR检测马尔尼菲篮状菌病组织样本中菌载量的研究
Chinese Journal of Mycology（2018）
Abstract
目的 实时荧光定量PCR测定马尔尼菲篮状菌病组织样本中马尔尼菲篮状菌的载量.方法 收集马尔尼菲篮状菌病患者的皮肤病理切片、淋巴结活检组织,提取总DNA,采用实时荧光定量PCR技术检测样本中马尔尼菲篮状菌载量.结果 皮肤病理切片、淋巴结活检组织中马尔尼菲篮状菌载量阳性.病理切片平均菌载量3.01×104 copies (1.07×104～7.26×104 copies),淋巴结活检组织中马尔尼菲篮状菌载量4.68×105 copies.结论 荧光定量PCR能高效检测皮肤病理切片、淋巴结活检组织中马尔尼菲篮状菌载量,对马尔尼菲篮状菌病诊断有一定的价值.
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Key words
talaromycosis marneffei (TSM),qPCR,diagnosis,pathological slices,biopsy tissues
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