表达新城疫病毒F基因重组鸭肠炎病毒的构建

Chinese Journal of Preventive Veterinary Medicine(2016)

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Abstract
为构建表达鸡新城疫病毒(NDV)F基因的重组鸭肠炎病毒(DEV),本研究以实验室前期构建的表达绿色荧光蛋白的重组DEV (rDEV-US 10-EGFP)为基础,通过PCR扩增NDV F基因,构建了携带F基因表达盒的转移质粒pUS 10-TPA-F.将转移质粒与rDEV-US 10-EGFP基因组DNA共转染鸡胚成纤维细胞.经过反向蚀斑筛选,获得表达NDV F基因的rDEV-US 10-F.对重组区域US10基因测序结果表明,F基因正确插入至US 10基因编码区内.将重组病毒传至15代,F基因仍能够稳定表达,表明重组病毒具有稳定的遗传特性,并且与亲本病毒株具有相似的生长曲线.本研究为研发NDV-DEV活载体疫苗奠定了基础.
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Key words
Newcastle disease virus,F gene,duck enteritis virus,recombinant virus
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