冻干保护剂对假单胞菌菌粉存活率的影响
Biotechnology Bulletin（2017）
Abstract
保护剂的添加对提高假单胞菌冷冻干燥菌粉的存活率和贮藏稳定性有显著效果.设计实验对冻干保护剂进行筛选和优化研究,通过单因素实验和正交实验筛选出了最佳保护剂组合:脱脂乳粉10%,山梨醇3%,甘油1%,L-抗坏血酸钠2%,假单胞菌冻干后的存活率可达90%,菌粉25℃存放6个月,存活率为50.2%.
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Key words
survival rate,cryoprotectant,Pseudomonas sp.
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