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人内皮素受体A基因克隆及表达

潘晓菲,时丽丽,谭初兵,吕超君,徐为人,汤立达

Biotechnology Bulletin（2014）

Cited 0|Views7
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Abstract
为了建立内皮素受体拮抗剂筛选系统,克隆人ETA基因,构建真核表达载体,并实现pTag-Lite SNAP-ETA在CHO-K1细胞中的瞬时表达.从人肺腺癌细胞系A549中克隆人ETA基因,连接到pTag-Lite SNAP质粒,构建表达载体pTag-Lite SNAP-ETA,用FuGENER HD转染试剂将表达载体pTag-Lite SNAP-ETA转染入CHO-K1细胞内,通过荧光显微镜检测融合蛋白SNAP-ETA的表达.DNA测序结果表明pTag-Lite SNAP-ETA表达载体构建成功,荧光显微镜检测结果表明人ETA在CHO-K1细胞中有效表达.
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