重组人成骨生长肽的表达、纯化和活性的研究
CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY(2004)
Abstract
将人工合成的人成骨生长肽基因(hOGP gene),与质粒pTYB2重组,转化大肠杆菌E.coliBL21(DE3).经异丙基硫代-β-D-半乳糖苷(IPTG)诱导,融合蛋白在E.coli中得到表达.菌体经超声破碎,一步亲和层析纯化得到重组人成骨生长肽(recombinant human osteogenic growth peptide,rhOGP).体外实验证明:rhOGP对成纤维细胞(NIH3T3)的增殖有促进作用.体内实验证明:rhOGP加速兔移植骨成活,使血清中碱性磷酸酶活性增高,骨钙素增高.重组人成骨生长肽促进成骨,有可能成为治疗骨移植及骨折愈合的有效药物.
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