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蚓激酶的克隆及其对BHK细胞的作用

孙兆军,梁国栋,陈飞,付士红, 沈悦,柴玉波,李晓宇,徐义辉,侯云德

CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY（2002）

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Abstract
蚯蚓具有活血化瘀的功效,在我国作为中药(地龙)使用已经有上千年的历史.近年来发现蚯蚓体内存在溶血栓成分,称为蚓激酶(lumbrukinase)或蚯蚓纤溶酶(thrombolytic enzyme in earthWorm).自N.Naka-jima报道从蚯蚓中分离到蚓激酶以来[1],国内外已经有许多报道[2-6],主要侧重于天然提取物的生化研究.本文通过RT-PCR从蚯蚓(L.bimastus)体内获得蚓激酶基因,进行表达并观察其对BHK细胞的作用.
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