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弧菌菌株发酵工艺初步研究

Jia-fa TAO,Ying-tiao LAI,Yan REN,Guang-hui KANG,ZHANG-You,Cun-bin SHI,Shu-qin WU

INDUSTRIAL MICROBIOLOGY（2011）

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Abstract
采用摇瓶培养和发酵罐培养并在不同培养时间测定菌液浊度和活菌数,为弧菌灭活疫苗生产工艺提供参数.研究结果表明,摇瓶培养装量以30%较为合适,培养基初始pH 7.5,发酵过程以不再控制为宜.初步确定了弧菌发酵培养工艺参数:弧菌菌株接种TSB,28 ℃摇瓶培养18～20 h,28 ℃种子罐通气搅拌培养12～14 h,28 ℃发酵罐通气搅拌培养12～14 h.
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