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短小芽孢杆菌2080碱性蛋白酶的纯化与性质

Zu-ming LI,Hong-yu LI,De-liang WANG,Xin MO,Zhi-hui BAI,Li-yun LI

INDUSTRIAL MICROBIOLOGY（2009）

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Abstract
短小芽孢杆菌(Bacillus pumilus)2080碱性蛋白酶的发酵液经超滤、硫酸铵沉淀、CM Sepharose Fast Flow和DEAE Sepharose Fast Flow离子交换层析得到了纯化的组分.SDS-PAGE电泳分析显示其分子量约为61 kDa.酶学性质研究表明,该纯化酶的最适 pH 为10.5,最适温度为50 ℃.
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