下调lincRNA HOTAIR对胃癌细胞迁移及增殖抑制作用的研究
Chinese Journal of Cell Biology（2015）
Abstract
通过下调人胃癌细胞BGC823中lincRNA HOTAIR基因的表达,该文探讨了lincRNAHOTAIR低表达对胃癌细胞迁移、侵袭及增殖能力的影响.该文构建针对人lincRNA HOTAIR基因的干扰质粒shHOTAIR,稳定转染入胃癌细胞BGC823、筛选稳转株,qPCR检测lincRNA HOTAIR在胃癌细胞中表达水平.采用划痕试验、侵袭试验、MTT法分别检测转染胃癌细胞迁移、侵袭及增殖能力.结果表明,稳定转染干扰质粒shHOTAI后下调lincRNA HOTAIR表达的胃癌细胞株细胞迁移、侵袭及增殖能力较阴性对照组明显减弱.下调胃癌细胞中lincRNA HOTAIR的表达,可降低胃癌细胞的迁移力、侵袭性、抑制其增殖能力,提示lincRNA HOTAIR可作为分子靶点用于胃癌的分子靶向治疗.
MoreKey words
gastric cancer,lincRNA HOTAIR,RNA interference,targeted therapy
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